Nikon N-STORM
Stoechastic Optical Reconstruction Microscopy (STORM) – a microscopy technique where single individual fluorochromes are localized over time, and from their positions a high-resolution image is reconstructed in 2D and 3D. Super-resolution image with 10 times higher resolution than conventional microscopy. Through this technique, molecular interactions at the nanoscale can be visualized.
Nikon N-STORM
Stoechastic Optical Reconstruction Microscopy (STORM) – a microscopy technique where single individual fluorochromes are localized over time, and from their positions a high-resolution image is reconstructed in 2D and 3D. Super-resolution image with 10 times higher resolution than conventional microscopy. Through this technique, molecular interactions at the nanoscale can be visualized.
Stoechastic Optical Reconstruction Microscopy (STORM) is a microscopy technique that localizes single individual fluorochromes over time, and from their position reconstructs a high-resolution image in 2D and 3D, a super-resolution image with 10 times higher resolution than conventional microscopy. Through this technique, one can visualize and obtain information about molecular interactions at the nanoscale.
Nikon has developed N-STORM with new optics that create more efficient illumination optimized for CMOS cameras. It provides 10 times faster image capture compared to previous systems and makes it possible to create STORM images even of living cells. In addition to the improved imaging speed, it is now also possible to expand the imaged field of view to 512 x 512 pixels. N-STORM together with the ultra-stable inverted microscope Ti2-E, and the tools in the NIS-Elements software create a versatile microscope system that gives the user unprecedented flexibility for many different applications.
Tenfold improvement in lateral resolution (to 20nm)
N-STORM uses localization information from thousands of individual fluorochromes to create stunning super-resolution images. It is possible to obtain an optical resolution that is 10 times higher than conventional optical microscopes.
Tenfold improvement in axial resolution (to 50 nm)
In addition to improving the lateral resolution, it is also possible to obtain a 3D STORM image with a tenfold improvement in axial resolution. In order to image large volumes, N-STORM has a 3D Stack function where several 3D STORM images in different Z-positions are combined into one image.
Dynamic Super Resolution
Imaging speed has been significantly improved, from minutes to seconds per image, thanks to newly developed optics and illumination optimized for sCMOS cameras. Thanks to this improvement, it is now possible to image living cells with a resolution 10 times greater than with conventional optical microscopes.
Multiple colors for protein interaction studies
To study interactions between multiple proteins, STORM images can be acquired with up to 3 fluorochromes, either by the sequential activator-reporter pair method, or with continuous activation of the fluorochromes.
Higher density of molecules gives higher resolution
The newly developed optics and the improved excitation capacity allow more molecules to be localized per image. This gives a more precise localization of molecules and thus higher resolution.
Correlative Superresolution for molecular information
N-STORM can be combined with confocal microscopy, for example with Nikon’s A1R HD25 confocal system. From a large field of view confocal image, you select an area of interest and switch to super-resolution with a click in the software. The two images are merged into a composite image where the confocal image provides structural information about the local environment of the molecules, and the super-resolution image provides information about the interaction of the proteins.
Contact the product specialist:
Catherine Kitts |
Marie Andersson |
Technical specification
XY resolution: about 20 nm
Z-resolution: about 50 nm
Methods: 2D-STORM (continuous or activated), 3D-STORM (continuous or activated), 3D-Stack
Frame rate: up to 500 Hz
Maximum FOV: 80 µm x 80 µm
Laser unit: LU-NV series Standard: 405 nm, 488 nm, 561 nm, 647 nm Option: 445 nm, 458 nm
Microscope: inverted microscope Eclipse Ti2-E
Camera: Hamamatsu Orca Flash 4.0 CMOS, Andor iXON Ultra EMCCD
Software: NIS-Elements AR
Stoechastic Optical Reconstruction Microscopy (STORM) is a microscopy technique that localizes single individual fluorochromes over time, and from their position reconstructs a high-resolution image in 2D and 3D, a super-resolution image with 10 times higher resolution than conventional microscopy. Through this technique, one can visualize and obtain information about molecular interactions at the nanoscale.
Nikon has developed N-STORM with new optics that create more efficient illumination optimized for CMOS cameras. It provides 10 times faster image capture compared to previous systems and makes it possible to create STORM images even of living cells. In addition to the improved imaging speed, it is now also possible to expand the imaged field of view to 512 x 512 pixels. N-STORM together with the ultra-stable inverted microscope Ti2-E, and the tools in the NIS-Elements software create a versatile microscope system that gives the user unprecedented flexibility for many different applications.
Tenfold improvement in lateral resolution (to 20nm)
N-STORM uses localization information from thousands of individual fluorochromes to create stunning super-resolution images. It is possible to obtain an optical resolution that is 10 times higher than conventional optical microscopes.
Tenfold improvement in axial resolution (to 50 nm)
In addition to improving the lateral resolution, it is also possible to obtain a 3D STORM image with a tenfold improvement in axial resolution. In order to image large volumes, N-STORM has a 3D Stack function where several 3D STORM images in different Z-positions are combined into one image.
Dynamic Super Resolution
Imaging speed has been significantly improved, from minutes to seconds per image, thanks to newly developed optics and illumination optimized for sCMOS cameras. Thanks to this improvement, it is now possible to image living cells with a resolution 10 times greater than with conventional optical microscopes.
Multiple colors for protein interaction studies
To study interactions between multiple proteins, STORM images can be acquired with up to 3 fluorochromes, either by the sequential activator-reporter pair method, or with continuous activation of the fluorochromes.
Higher density of molecules gives higher resolution
The newly developed optics and the improved excitation capacity allow more molecules to be localized per image. This gives a more precise localization of molecules and thus higher resolution.
Correlative Superresolution for molecular information
N-STORM can be combined with confocal microscopy, for example with Nikon’s A1R HD25 confocal system. From a large field of view confocal image, you select an area of interest and switch to super-resolution with a click in the software. The two images are merged into a composite image where the confocal image provides structural information about the local environment of the molecules, and the super-resolution image provides information about the interaction of the proteins.
Contact the product specialist:
|
Catherine Kitts |
Marie Andersson |
Technical specification
XY resolution: about 20 nm
Z-resolution: about 50 nm
Methods: 2D-STORM (continuous or activated), 3D-STORM (continuous or activated), 3D-Stack
Frame rate: up to 500 Hz
Maximum FOV: 80 µm x 80 µm
Laser unit: LU-NV series Standard: 405 nm, 488 nm, 561 nm, 647 nm Option: 445 nm, 458 nm
Microscope: inverted microscope Eclipse Ti2-E
Camera: Hamamatsu Orca Flash 4.0 CMOS, Andor iXON Ultra EMCCD
Software: NIS-Elements AR
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