Nikon CFI60 Silicone Immersion Series
Nikon silicone objectives; designed for microscopy of thick samples such as 3D cultures, organoids and tissue cultures.
Nikon CFI60 Silicone Immersion Series
Nikon silicone objectives; designed for microscopy of thick samples such as 3D cultures, organoids and tissue cultures.
See full 3D cell cultures, organoids and tissues in high resolution
Nikon’s silicone objectives are corrected for use with silicone as the immersion between objective and sample.
Silicone with a refractive index of ~1.4 matches biological and tissue samples well, and the long working distance makes the lenses ideal for microscopy of 3D cell cultures and thick tissue samples.
The silicone lenses also have high numerical aperture and enable visualization of cellular dynamics with high S/N even deep inside the sample.
Observe live samples for long periods of time without having to add more immersion
Silicone oil is durable and does not evaporate quickly.
It is also stable at the higher temperatures required for live cell cultures, for example.
This allows longer time-lapse series to be made without having to add more immersion (as is necessary with water as a medium, for example).
The series is available in several magnifications (25X, 40X, 60X and 100X) so it is possible to switch from low magnification to high magnification without having to clean and change the medium.
Newly developed glass with high degree of correction
Nikon is the only supplier that manufactures its own glass and has developed a new glass (short-wavelength refractive) with the silicon series that reduces the chromatic aberration for short wavelengths.
This extends the spectral range that can be used without aberrations.
In addition, the silicone lenses, like all lenses in the Lambda series, are treated with “Nano Crystal Coat”, an anti-reflective surface that dramatically reduces internal reflections.
Contact the product specialist:
 Catherine Kitts catherine.kitts@bergmanlabora.se Tel: 031-788 18 94 |
 Marie Andersson marie.andersson@bergmanlabora.se Tel: 08-625 18 07 |
Technical specification
- CFI Plan Apochromat Lambda S 25XC Sil, NA 1.05, WD 0.55 mm, correction for cover glass: 0.11 – 0.23
- CFI Plan Apochromat Lambda S 40XC Sil, NA 1.25, WD 0.3 mm, cover glass correction: 0.13 – 0.21 (23˚C) 0.15 – 0.23 (37˚C)
- CFI Plan Apochromat Lambda S 60XC Sil, NA 1.30, WD 0.3 mm, correction for cover glass: 0.15 – 0.19
- CFI SR HP Plan Apochromat Lambda S 100X Sil, NA 1.35, WD 0.30* (0.31 – 0.29) (23˚C) 0.29* (0.30 – 0.28) (37˚C), correction for cover glass: 0.15 – 0.19
See full 3D cell cultures, organoids and tissues in high resolution
Nikon’s silicone objectives are corrected for use with silicone as the immersion between objective and sample.
Silicone with a refractive index of ~1.4 matches biological and tissue samples well, and the long working distance makes the lenses ideal for microscopy of 3D cell cultures and thick tissue samples.
The silicone lenses also have high numerical aperture and enable visualization of cellular dynamics with high S/N even deep inside the sample.
Observe live samples for long periods of time without having to add more immersion
Silicone oil is durable and does not evaporate quickly.
It is also stable at the higher temperatures required for live cell cultures, for example.
This allows longer time-lapse series to be made without having to add more immersion (as is necessary with water as a medium, for example).
The series is available in several magnifications (25X, 40X, 60X and 100X) so it is possible to switch from low magnification to high magnification without having to clean and change the medium.
Newly developed glass with high degree of correction
Nikon is the only supplier that manufactures its own glass and has developed a new glass (short-wavelength refractive) with the silicon series that reduces the chromatic aberration for short wavelengths.
This extends the spectral range that can be used without aberrations.
In addition, the silicone lenses, like all lenses in the Lambda series, are treated with “Nano Crystal Coat”, an anti-reflective surface that dramatically reduces internal reflections.
Contact the product specialist:
| Catherine Kitts catherine.kitts@bergmanlabora.se Tel: 031-788 18 94 |
Marie Andersson marie.andersson@bergmanlabora.se Tel: 08-625 18 07 |
Technical specification
- CFI Plan Apochromat Lambda S 25XC Sil, NA 1.05, WD 0.55 mm, correction for cover glass: 0.11 – 0.23
- CFI Plan Apochromat Lambda S 40XC Sil, NA 1.25, WD 0.3 mm, cover glass correction: 0.13 – 0.21 (23˚C) 0.15 – 0.23 (37˚C)
- CFI Plan Apochromat Lambda S 60XC Sil, NA 1.30, WD 0.3 mm, correction for cover glass: 0.15 – 0.19
- CFI SR HP Plan Apochromat Lambda S 100X Sil, NA 1.35, WD 0.30* (0.31 – 0.29) (23˚C) 0.29* (0.30 – 0.28) (37˚C), correction for cover glass: 0.15 – 0.19
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