Nikon AX / AX R with NSPARC
Market-leading 25mm field of view, 8k x 8k resolution and 30 frames per second (2048 x 512) characterize Nikon’s 10th generation (Point Scanning) confocal. Simply put, deliver more data faster!
Nikon AX / AX R with NSPARC
Market-leading 25mm field of view, 8k x 8k resolution and 30 frames per second (2048 x 512) characterize Nikon’s 10th generation (Point Scanning) confocal. Simply put, deliver more data faster!
High resolution and wide field of view in one system
Nikon is the world’s leading manufacturer of confocal microscopes that combine high resolution with a large field of view. With the Nikon AX and AX R confocal systems, images of up to 8192 x 8192 pixels can be captured with a field of view of 25 mm with both the Ti2 inverted microscope and the Ni and FN1 upright microscopes. This allows you to see, for example, whole mouse brains or whole Drosophila embryos in a single field of view at low magnification and still have sufficient resolution to see small details. The large field of view also helps to speed up image analysis as more data can be collected in a single field of view.
Speed and high resolution for dynamic events
Nikon’s AXR system features an innovative hybrid scanner that includes a fast galvanometer scanner and an ultra-fast resonant scanner. The hybrid scanner allows you to switch between a frame rate of up to 30 frames per second at a resolution of 2048 x 2048 pixels to follow dynamic events in living cells and, if necessary, switch to higher resolution with the galvanometer scanner. Switching between the resonance scanner and the galvanic scanner is done by a simple click in the software.
Detectors for high sensitivity and spectral flexibility
Nikon’s DUX-VB and DUX-ST detectors can be equipped with 2 or 4 GaAsP or High QE PMT detectors. The detectors can be filterless for free spectral adjustability or with filters of up to 24 channels. The detectors can be combined to provide both spectral specificity and freedom of choice.
Nikon NSPARC – Revolutionary technology for improved resolution and image quality
Nikon’s latest technology, Nikon Spatial Array Confocal (NSPARC), is a detector that has been developed to further enhance the resolution and sensitivity of AX and AX R users. NSPARC contains an array of 25 super-sensitive detectors. Each individual detector receives two-dimensional information from each scanned point, as opposed to a standard detector that receives information from only one pixel. This method of image scanning, called Image Scanning Microscopy (ISM), provides higher sensitivity and improves the signal-to-noise ratio, which means you can reduce laser intensity.
In addition, the technology provides improved resolution of up to 80-100 nm laterally and 300 nm axially so that even small details that cannot be seen with a standard detector become visible. This new detector can be used as a stand-alone detector, but it can also be combined with DUX-VB or DUX-ST for maximum flexibility. It is also possible to upgrade existing AX/AXR confocals with NSPARC.
Simplify with new AI tools
AX/AXR’s control software is user-friendly. All the user needs to do is decide which fluorochromes to use and then let the AI set the levels of lasers and detectors for the best possible image quality. This can be further combined with NIS.ai which has several tools for automatic image enhancement and thresholding for image analysis.
AX and AX R confocal microscopes can be combined with other microscopy techniques
AX/AXR can be combined with other microscopy techniques such as SIM, TIRF, Spinning Disk, FLIM and FRAP. Our NIS-Elements software is flexible and can also be configured to control other hardware, such as pumps and micromanipulators to create more complex experiment designs.
Contact the product specialist:
Catherine Kitts |
Marie Andersson |
Technical specification
Scanning unit: 2 laser inputs, 2 signal outputs for standard, spectral and optional detectors, external excitation and emmersion ports
Galvano, 8192 x 8192 pixels, 10 fps @ 512-512 pixels
Resonant, 2048 x 2048 pixels, 30 fps (2048 x 512 pixels), to 720 fps (2048 x 16 pixels)
FOV: 25 mm
Pinhole: continuously variable
Laser: up to 8 lasers from 405 nm, to 750 nm, query for wavelengths
DUX-VB detectors: 2 or 4 channel detector (GaAsP or high sensitivity PMT) with free adjustability +/- 1 nm and up to 66 channels, up to 12 bandpass filters
DUX-ST detectors: 2 or 4 channel detector (GaAsP, high sensitivity PMT) with up to 24-bandpass filter Transmission detector
Microscope: Ti2-E, Ni-E, FN1
Software: NIS-Elements C
High resolution and wide field of view in one system
Nikon is the world’s leading manufacturer of confocal microscopes that combine high resolution with a large field of view. With the Nikon AX and AX R confocal systems, images of up to 8192 x 8192 pixels can be captured with a field of view of 25 mm with both the Ti2 inverted microscope and the Ni and FN1 upright microscopes. This allows you to see, for example, whole mouse brains or whole Drosophila embryos in a single field of view at low magnification and still have sufficient resolution to see small details. The large field of view also helps to speed up image analysis as more data can be collected in a single field of view.
Speed and high resolution for dynamic events
Nikon’s AXR system features an innovative hybrid scanner that includes a fast galvanometer scanner and an ultra-fast resonant scanner. The hybrid scanner allows you to switch between a frame rate of up to 30 frames per second at a resolution of 2048 x 2048 pixels to follow dynamic events in living cells and, if necessary, switch to higher resolution with the galvanometer scanner. Switching between the resonance scanner and the galvanic scanner is done by a simple click in the software.
Detectors for high sensitivity and spectral flexibility
Nikon’s DUX-VB and DUX-ST detectors can be equipped with 2 or 4 GaAsP or High QE PMT detectors. The detectors can be filterless for free spectral adjustability or with filters of up to 24 channels. The detectors can be combined to provide both spectral specificity and freedom of choice.
Nikon NSPARC – Revolutionary technology for improved resolution and image quality
Nikon’s latest technology, Nikon Spatial Array Confocal (NSPARC), is a detector that has been developed to further enhance the resolution and sensitivity of AX and AX R users. NSPARC contains an array of 25 super-sensitive detectors. Each individual detector receives two-dimensional information from each scanned point, as opposed to a standard detector that receives information from only one pixel. This method of image scanning, called Image Scanning Microscopy (ISM), provides higher sensitivity and improves the signal-to-noise ratio, which means you can reduce laser intensity.
In addition, the technology provides improved resolution of up to 80-100 nm laterally and 300 nm axially so that even small details that cannot be seen with a standard detector become visible. This new detector can be used as a stand-alone detector, but it can also be combined with DUX-VB or DUX-ST for maximum flexibility. It is also possible to upgrade existing AX/AXR confocals with NSPARC.
Simplify with new AI tools
AX/AXR’s control software is user-friendly. All the user needs to do is decide which fluorochromes to use and then let the AI set the levels of lasers and detectors for the best possible image quality. This can be further combined with NIS.ai which has several tools for automatic image enhancement and thresholding for image analysis.
AX and AX R confocal microscopes can be combined with other microscopy techniques
AX/AXR can be combined with other microscopy techniques such as SIM, TIRF, Spinning Disk, FLIM and FRAP. Our NIS-Elements software is flexible and can also be configured to control other hardware, such as pumps and micromanipulators to create more complex experiment designs.
Contact the product specialist:
|
Catherine Kitts |
Marie Andersson |
Technical specification
Scanning unit: 2 laser inputs, 2 signal outputs for standard, spectral and optional detectors, external excitation and emmersion ports
Galvano, 8192 x 8192 pixels, 10 fps @ 512-512 pixels
Resonant, 2048 x 2048 pixels, 30 fps (2048 x 512 pixels), to 720 fps (2048 x 16 pixels)
FOV: 25 mm
Pinhole: continuously variable
Laser: up to 8 lasers from 405 nm, to 750 nm, query for wavelengths
DUX-VB detectors: 2 or 4 channel detector (GaAsP or high sensitivity PMT) with free adjustability +/- 1 nm and up to 66 channels, up to 12 bandpass filters
DUX-ST detectors: 2 or 4 channel detector (GaAsP, high sensitivity PMT) with up to 24-bandpass filter Transmission detector
Microscope: Ti2-E, Ni-E, FN1
Software: NIS-Elements C
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